Milk Fraud/Bovine Rennet Whey ELISA
Cat. No.: 5171BRW
Bovine rennet whey (BRW) is a low-priced by-product of cheese production from bovine milk of which most is traded as powder (BRWp). Due to its low price, fraudulent mixing with the higher priced bovine milk and especially with the milk of other species (e.g. goat, sheep) and sources (e.g. soy, almond) is economically attractive. However, this is illegal, dangerous for allergic consumers and can lead to quality problems in end products such as yoghurt and infant formulas.
Additions of BRW to dairy and other products can be detected by the presence of the bovine glycomacropeptide (GMP), which is released from bovine κ-casein by enzymatic (rennet) hydrolysis during cheese preparation. The BRW ELISA uses a specific monoclonal antibody which recognizes a 5 amino acids-containing epitope on the GMP part of bovine κ-casein.
For the detection of added BRWp to milk of other species and sources, milk samples are added directly to the test after a dilution in buffer, resulting in limits of detection (LODs) of 0.03% BRWp (1:100 diluted) and 0.3% BRWp (1:1000 diluted). For the detection of added BRWp to bovine milk, the bovine’s κ-casein needs to be removed which is done by precipitation with trichloroacetic acid (TCA). The GMP containing neutralized supernatant is analysed after a dilution in buffer which results in an LOD of 0.2% BRWp.
Detailed information of this ELISA is given in the Product Information Sheet
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