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Corticosteroid ELISA

Cat. No.: 5081COR 

Synthetic corticosteroids are widely used in live-stock breeding to treat inflammatory diseases. The Maximum Residue Limits (MRLs) for dexamethasone and betamethasone in milk, tissue, kidney and liver are 0.3, 0.75, 0.75 and 2.0 µg/kg, respectively. For prednisolone the MRLs are 4, 4, 6, 10 and 10 µg/kg for muscle, fat, milk, liver and kidney, respectively. For methylprednisolone the MRLs are 10 µg/kg for each of the matrices mentioned. Although some corticosteroids are permitted for use as drugs, corticosteroids in general are used as growth promoters in meat production. The use of growth promoters in the European Community was banned since 1986. In order to detect Corticosteroids a competitive enzyme immunoassay for screening feed, milk and urine for the following corticoste­roids: flumethasone (125%), dexamethasone (100%), betametha­sone (60%), triamcinolone (33%), prednisolone (21%), dianabol (7.5%) and methylprednisolone (2.8%) is available. The measuring range of dexamethasone begins from 0.125 – 4 ng/ml. Milk can be used directly in this test with a sensitivity as low as 0.15 ng/ml. However, feed samples, undergo a sample preparation procedure as described in the manual and the sensitivity is down to 20 ng/g. Urine samples can be applied in the assay after a dilution step in buffer or after an HPLC fractionati­on. Sample preparation procedures for other matrices are explained in the kit manual. Detailed information of this ELISA is given in the Product Information Sheet.

Product information sheet COR[5]16Download
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