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Chloramphenicol ELISA

General

The Chloramphenicol (CAP) ELISA detects CAP and its major metabolite (CAP-glucuronide) in a variety of matrices. This kit has a unique sensitivity, robustness and reproducibility, which has resulted in a leading market position for already several years. The Chloramphenicol ELISA is a competitive enzyme immunoassay based on antibodies directed against chloramphenicol.

Kit characteristics

Microtiter plate
96 wells
12 x 8 Breakapart
Antibody cross-reactivity
CAP glucuronide 65%
CAP-base < 1%
Thiamphenicol < 1%
Florphenicol < 1%
Conjugate
CAP-HRP stabilized
Standard range (ready-to-use)
0, 0.025, 0.05, 0.1, 0.2, 0.5 and 2.0 ng/ml


Assay characteristics

Matrices LOD (ppb)
Milk 0.1
Urine 0.01
Tissue*, egg, honey 0.02
Liver 0.01
Feed 0.1
* Tissue: meat, crab, shrimp, fish.

The Limit of detection (LOD) is calculated as: Xn+3SD and is determined under optimal conditions.

Sample preparation

For milk, urine, tissue (meat, crab, shrimp, fish), egg, honey, liver and feed fast and efficient methods are included in the kit manual.

Procedure

Antibody, conjugate and sample/standard are pipetted into the wells and incubated for one hour at 2°C – 8°C. After a washing procedure ready-to-use substrate is added and incubated for 30 minutes at 20°C – 25°C. The reaction is stopped and the absorbance is read in a spectrophotometer at 450 nm.

EuroProxima’s user-friendly software converts the measured optical density into the concentration of the metabolite in the starting material.

Chloramphenicol ELISA EuroProxima

Downloads 
Product information sheet CAP[5]15Download
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