The Chloramphenicol (CAP) ELISA detects CAP and its major metabolite (CAP-glucuronide) in a variety of matrices. This kit has a unique sensitivity, robustness and reproducibility, which has resulted in a leading market position for already several years. The Chloramphenicol ELISA is a competitive enzyme immunoassay based on antibodies directed against chloramphenicol.
12 x 8 Breakapart
Standard range (ready-to-use)
0, 0.025, 0.05, 0.1, 0.2, 0.5 and 2.0 ng/ml
Tissue*, egg, honey
* Tissue: meat, crab, shrimp, fish.
The Limit of detection (LOD) is calculated as: Xn+3SD and is determined under optimal conditions.
For milk, urine, tissue (meat, crab, shrimp, fish), egg, honey, liver and feed fast and efficient methods are included in the kit manual.
Antibody, conjugate and sample/standard are pipetted into the wells and incubated for one hour at 2°C – 8°C. After a washing procedure ready-to-use substrate is added and incubated for 30 minutes at 20°C – 25°C. The reaction is stopped and the absorbance is read in a spectrophotometer at 450 nm.
EuroProxima’s user-friendly software converts the measured optical density into the concentration of the metabolite in the starting material.