A flow-through enzyme immunoassay for the detection of Ochratoxin-A in green coffee.
The Ochratoxin-A (OTA) Flow-Through Rapid Test is a competitive membrane-based enzyme immunoassay for the detection of OTA in green coffee. The test is based on mouse monoclonal antibodies against OTA. With this kit 10 analyses can be performed. The kit contains all the reagents required to perform the tests. The cut-off value of this test is 3 μg/kg of OTA in green coffee.
Ochratoxin A (OTA) is a nephrotoxic and hepatocarcinogenic mycotoxin produced by Penicillium verrucosum and Penicillium viridicatum in temperate and cold climates and by a number of Aspergillus species such as A. ochraceus in warmer and tropical areas of the world. OTA has been shown to occur in various cereals and plant products such as coffee and grapes. In the European Union the maximum limit for OTA in roasted coffee is 3 μg/kg in accordance with Commission Regulation (EU) 2022/1370. There is no maximum limit set for green coffee. In line with regulation for roasted coffee the cut-off value for green coffee was set to 3 μg/kg.