Oxolinic acid is a synthetic antimicrobial agent belonging to the first quinolone class of antibiotics. Quinolones are generally used in veterinary medicine for treatment of cattle, pigs, poultry, fish and shrimp. The specific use of Oxolinic acid is mostly reported in aquaculture. After antibiotic treatment, the level of residues in animal derived products should be low to limit human exposure to these drug residues. Therefore, maximum residue limits (MRLs) are set for quinolones in several regions of the world.

In the European Union in accordance with Commission Regulation (EU) No 37/2010 the maximum residue limits (MRLs) for oxolinic acid in animal derived products are 100 µg/kg in muscle, 50 µg/kg in fat, 150 µg/kg in liver and 150 µg/kg in kidney. For fish, prawn and shrimp the legislation is more stringent. In Thailand, for example, a maximum residue limit of 5 µg/kg is in force for sea food.

R-Biopharm Nederland has developed a specific ELISA test for oxolinic acid. This test completes the EuroProxima branded portfolio for the screening of quinolones. For this purpose, there were already 2 generic tests, as well as specific tests for respectively enrofloxacin and flumequine available.

Now the 5101OXO Oxolinic acid ELISA has been brought on the market. This competitive ELISA test has a total incubation time of 1 hour and comes with a simple and fast sample preparation. The detection capability of this test is determined at 3 µg/kg for fish, prawn and shrimp.

For additional information about this new test, please contact us at [email protected]